Beaconsfield SYCOB, Chesham United, Egham Town, Kettering Town, Potters Bar Town, North Leigh, Taunton Town and Weymouth are all bidding to book a place in the first round proper and collect another £12,500 in prize money as fourth and final qualifying round winners.
In a message on Twitter, the EVO-STIK League Southern said: "Good luck and best wishes to all our clubs in the Emirates FA Cup today, whatever your results you have already done us proud."
Follow the EVO-STIK League Southern on Twitter at @SouthernLeague1
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