The fans, players and officials of Chesham United and Taunton Town will be looking out for balls 64 and 67 respectively when the draw is broadcast live on BBC2 and BT Sport 1. Both programmes start at 7pm with the draw expected to start at approximately 7.10pm.
To make it into the hat, Chesham United beat EVO-STIK League Southern rivals Potters Bar Town 1-0 at home while Taunton Town must go again this week with Hemel Hempstead Town after holding the National League South side to a 0-0 draw at home.
After collecting £12,500 as fourth qualifying round winners for the second season in a row, the Generals, and Taunton if they make it through, are in the running to bank another £18,000 in prize money as well as televised ties.
The first round proper is due to be played over the weekend of Saturday November 5 and Sunday November 6 with 80 clubs going into the hat - the 32 winners of last weekend's fourth qualifying round plus the 48 teams from League One and League Two.
Neutrals will be keeping an eye out for ball number 62 of Hereford-based Westfields FC, the lowest ranked side left in the competition from the Midland Football League.
First round draw numbers
1 Accrington Stanley
2 AFC Wimbledon
3 Barnet
4 Blackpool
5 Bolton Wanderers
6 Bradford City
7 Bristol Rovers
8 Bury
9 Cambridge United
10 Carlisle United
11 Charlton Athletic
12 Cheltenham Town
13 Chesterfield
14 Colchester United
15 Coventry City
16 Crawley Town
17 Crewe Alexandra
18 Doncaster Rovers
19 Exeter City
20 Fleetwood Town
21 Gillingham
22 Grimsby Town
23 Hartlepool United
24 Leyton Orient
25 Luton Town
26 Mansfield Town
27 Millwall
28 Milton Keynes Dons
29 Morecambe
30 Newport County
31 Northampton Town
32 Notts County
33 Oldham Athletic
34 Oxford United
35 Peterborough United
36 Plymouth Argyle
37 Port Vale
38 Portsmouth
39 Rochdale
40 Scunthorpe United
41 Sheffield United
42 Shrewsbury Town
43 Southend United
44 Stevenage
45 Swindon Town
46 Walsall
47 Wycombe Wanderers
48 Yeovil Town
49 Southport
50 Alfreton Town or Gateshead
51 Macclesfield Town
52 FC Halifax Town
53 Stockport County
54 Barrow
55 Stourbridge
56 Altrincham
57 Lincoln City or Guiseley
58 Spennymoor Town
59 Wrexham or Stamford
60 York City or Curzon Ashton
61 Whitehawk
62 Westfields
63 Sutton United
64 Chesham United
65 Dagenham & Redbridge
66 Torquay United or Woking
67 Taunton Town or Hemel Hempstead Town
68 Braintree Town
69 Brackley Town
70 Dartford
71 St Albans City
72 Boreham Wood
73 Eastbourne Borough
74 Harrow Borough or Margate
75 Maidstone United
76 Kidderminster Harriers
77 Solihull Moors
78 Dover Athletic
79 Merstham
80 Eastleigh
Follow us: @evostikleague on Twitter | Facebook www.facebook.com/evostikleague | The NPL Facebook Group www.facebook.com/groups/TheNPL/
Keep up-to-date with our exclusive email newsletters.
Subscribe